Skip navigation

Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.contributor.authorGuzik, Urszula-
dc.contributor.authorHupert-Kocurek, Katarzyna-
dc.contributor.authorKrysiak, Marta-
dc.contributor.authorWojcieszyńska, Danuta-
dc.identifier.citationBioMed Research International, Vol. 2014 (2014), art. no. 138768pl_PL
dc.description.abstractMicrobial intradiol dioxygenases have been shown to have a great potential for bioremediation; however, their structure is sensitive to various environmental and chemical agents. Immobilization techniques allow for the improvement of enzyme properties. This is the first report on use of glyoxyl agarose and calciumalginate asmatrixes for the immobilization of protocatechuate 3,4-dioxygenase. Multipoint attachment of the enzyme to the carrier caused maintenance of its initial activity during the 21 days. Immobilization of dioxygenase in calciumalginate or on glyoxyl agarose resulted in decrease in the optimumtemperature by 5∘Cand10∘C, respectively. Entrapment of the enzyme in alginate gel shifted its optimum pH towards high-alkaline pH while immobilization of the enzyme on glyoxyl agarose did not influence pHprofile of the enzyme. Protocatechuate 3,4-dioygenase immobilized in calciumalginate showed increased activity towards 2,5-dihydroxybenzoate, caffeic acid, 2,3-dihydroxybenzoate, and 3,5-dihydroxybenzoate. Slightly lower activity of the enzyme was observed after its immobilization on glyoxyl agarose. Entrapment of the enzyme in alginate gel protected it against chelators and aliphatic alcohols while its immobilization on glyoxyl agarose enhanced enzyme resistance to inactivation by metal ions.pl_PL
dc.rightsUznanie autorstwa 3.0 Polska*
dc.titleDegradation potential of protocatechuate 3,4-dioxygenase from crude extract of stenotrophomonas maltophilia strain KB2 immobilized in calcium alginate hydrogels and on glyoxyl agarosepl_PL
dc.relation.journalBioMed Research Internationalpl_PL
Appears in Collections:Artykuły (WBiOŚ)

Files in This Item:
File Description SizeFormat 
Guzik_Degradation_potential_of_protocatechuate.pdf1,48 MBAdobe PDFView/Open
Show simple item record

Uznanie Autorstwa 3.0 Polska Creative Commons License Creative Commons