DC pole | Wartość | Język |
dc.contributor.author | Majka, Joanna | - |
dc.contributor.author | Bzdęga, Katarzyna | - |
dc.contributor.author | Janiak, Agnieszka | - |
dc.contributor.author | Ćwiek-Kupczyńska, Hanna | - |
dc.contributor.author | Krajewski, Paweł | - |
dc.contributor.author | Książczyk, Tomasz | - |
dc.contributor.author | Zwierzykowski, Zbigniew | - |
dc.date.accessioned | 2019-05-31T08:37:54Z | - |
dc.date.available | 2019-05-31T08:37:54Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | BMC Genomics, Vol. 20 (2019), Art. No. 367 | pl_PL |
dc.identifier.issn | 1471-2164 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.12128/9263 | - |
dc.description.abstract | Background: Species of the Festuca and Lolium genera, as well as intergeneric Festuca × Lolium (Festulolium)
hybrids, are valuable fodder and turf grasses for agricultural and amenity purposes worldwide. Festulolium hybrids
can merge in their genomes agronomically important characteristics. However, in polyploid plants, especially in
allopolyploids, the hybridization of divergent genomes could contribute to various abnormalities, such as variability
in chromosome number, structural rearrangements, and/or disorders in inheritance patterns. Here we studied these
issues in allotetraploid Festuca pratensis × Lolium perenne hybrids.
Results: Cytogenetic procedures, including fluorescent in situ hybridization, genomic in situ hybridization, and
molecular markers – inter-simple sequence repeats (ISSR) were exploited. This cytogenetic approach indicated the
dynamics in the number and distribution of ribosomal RNA genes and structural rearrangements for both parental
genomes (Festuca and Lolium) in hybrid karyotypes. The separate analysis of F. pratensis and L. perenne
chromosomes in hybrid plants (F2-F3 generations of F. pratensis × L. perenne) revealed the asymmetrical level of
rearrangements. Recognized structural changes were mainly located in the distal part of chromosome arms, and in
chromosomes bearing ribosomal DNA, they were more frequently mapped in arms without this sequence. Based on
the ISSR markers distribution, we found that the tetrasomic type of inheritance was characteristic for the majority of
ISSR loci, but the disomic type was also observed. Nonetheless, no preference in the transmission of either Festuca or
Lolium alleles to the following generations of allotetraploid F. pratensis × L. perenne hybrid was observed.
Conclusion: Our study reports cytogenetic and molecular genotyping of the F. pratensis × L. perenne hybrid and its
following F2-F3 progenies. The analysis of 137 allotetraploid F. pratensis × L. perenne hybrids revealed the higher level of
recombination in chromosomes derived from F. pratensis genome. The results of ISSR markers indicated a mixed model
of inheritance, which may be characteristic for these hybrids. | pl_PL |
dc.language.iso | en | pl_PL |
dc.rights | Uznanie autorstwa 3.0 Polska | * |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/pl/ | * |
dc.subject | Festuca | pl_PL |
dc.subject | Festulolium hybrids | pl_PL |
dc.subject | Lolium | pl_PL |
dc.subject | Genomic composition | pl_PL |
dc.subject | Inheritance pattern | pl_PL |
dc.subject | Rearrangements | pl_PL |
dc.subject | Recombination | pl_PL |
dc.title | Cytogenetic and molecular genotyping in the allotetraploid Festuca pratensis × Lolium perenne hybrids | pl_PL |
dc.type | info:eu-repo/semantics/article | pl_PL |
dc.identifier.doi | 10.1186/s12864-019-5766-2 | - |
Pojawia się w kolekcji: | Artykuły (WNP)
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