DC pole | Wartość | Język |
dc.contributor.author | Kwaśniewska, Jolanta | - |
dc.contributor.author | Jaśkowiak, Joanna | - |
dc.date.accessioned | 2019-06-04T09:57:33Z | - |
dc.date.available | 2019-06-04T09:57:33Z | - |
dc.date.issued | 2016 | - |
dc.identifier.citation | PLoS ONE, 2016, no. 6, art. no. e0156865 | pl_PL |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.12128/9348 | - |
dc.description.abstract | In the present study, the combination of the micronucleus test with analysis of the activity of
the rRNA genes in mutagen-treated Hordeum vulgare (barley) by maleic hydrazide (MH)
cells was performed. Simultaneously fluorescence in situ hybridization (FISH) with 25S
rDNA as probes and an analysis of the transcriptional activity of 35S rRNA genes with silver
staining were performed. The results showed that transcriptional activity is always maintained
in the micronuclei although they are eliminated during the next cell cycle. The analysis
of the transcriptional activity was extended to barley nuclei. MH influenced the fusion of
the nucleoli in barley nuclei. The silver staining enabled detection of the nuclear bodies
which arose after MH treatment. The results confirmed the usefulness of cytogenetic techniques
in the characterization of micronuclei. Similar analyses can be now extended to
other abiotic stresses to study the response of plant cells to the environment | pl_PL |
dc.language.iso | en | pl_PL |
dc.rights | Uznanie autorstwa 3.0 Polska | * |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/pl/ | * |
dc.subject | heterochromatin | pl_PL |
dc.subject | maleic hydrazide | pl_PL |
dc.subject | RNA gene | pl_PL |
dc.title | Transcriptional activity of rRNA genes in barley cells after mutagenic treatment | pl_PL |
dc.type | info:eu-repo/semantics/article | pl_PL |
dc.relation.journal | PLoS ONE | pl_PL |
dc.identifier.doi | 10.1371/journal.pone.0156865 | - |
Pojawia się w kolekcji: | Artykuły (WNP)
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