DC pole | Wartość | Język |
dc.contributor.author | Juchimiuk-Kwaśniewska, Jolanta | - |
dc.contributor.author | Brodziak, L. | - |
dc.contributor.author | Małuszyńska, Jolanta | - |
dc.date.accessioned | 2019-06-06T12:39:21Z | - |
dc.date.available | 2019-06-06T12:39:21Z | - |
dc.date.issued | 2011 | - |
dc.identifier.citation | Journal of Applied Genetics, Vol. 52 (2011), s. 23-29 | pl_PL |
dc.identifier.issn | 1234-1983 | - |
dc.identifier.issn | 2190-3883 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.12128/9401 | - |
dc.description.abstract | A micronucleus test in combination with fluorescent
in situ hybridization (FISH) using telomere-,
centromere-specific probes and 5S and 25S rDNA was
used for a detailed analysis of the effects of gamma ray
irradiation on the root tip meristem cells of barley,
Hordeum vulgare (2n=14). FISH with four DNA probes
was used to examine the involvement of specific chromosomes
or chromosome fragments in gamma ray-induced
micronuclei formation and then to explain their origin.
Additionally, a comparison of the possible origin of the
micronuclei induced by physical and chemical treatment:
maleic hydrazide (MH) and N-nitroso-N-methylurea
(MNU) was done. The micronuclei induced by gamma
ray could originate from acentric fragments after chromosome
breakage or from whole lagging chromosomes as a
result of a dysfunction of the mitotic apparatus. No
micronuclei containing only centromeric signals were
found. An application of rDNA as probes allowed it to be
stated that 5S rDNA–bearing chromosomes are involved in
micronuclei formation more often than NOR chromosomes.
This work allowed the origin of physically- and chemicallyinduced
micronuclei in barley cells to be compared: the
origin of micronuclei was most often from terminal fragments.
FISH confirmed its usefulness in the characterization
of micronuclei content, as well as in understanding and
comparing the mechanisms of the actions of mutagens
applied in plant genotoxicity. | pl_PL |
dc.language.iso | en | pl_PL |
dc.rights | Uznanie autorstwa-Użycie niekomercyjne 3.0 Polska | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc/3.0/pl/ | * |
dc.subject | Barley | pl_PL |
dc.subject | FISH | pl_PL |
dc.subject | Gamma ray | pl_PL |
dc.subject | Micronuclei | pl_PL |
dc.title | FISH in analysis of gamma ray-induced micronuclei formation in barley | pl_PL |
dc.type | info:eu-repo/semantics/article | pl_PL |
dc.identifier.doi | 10.1007/s13353-010-0017-x | - |
Pojawia się w kolekcji: | Artykuły (WNP)
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