DC pole | Wartość | Język |
dc.contributor.author | Ledwoń, A. | - |
dc.contributor.author | Gaj, Małgorzata D. | - |
dc.date.accessioned | 2019-06-12T11:27:25Z | - |
dc.date.available | 2019-06-12T11:27:25Z | - |
dc.date.issued | 2011 | - |
dc.identifier.citation | Plant Growth Regulation, Vol. 65 (2011), s. 157-167 | pl_PL |
dc.identifier.issn | 0167-6903 | - |
dc.identifier.issn | 1573-5087 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.12128/9455 | - |
dc.description.abstract | The expression pattern of the LEC1 and FUS3
genes during somatic embryogenesis in Arabidopsis
explants (immature zygotic embryos) induced in vitro was
analysed, using Real-time quantitative PCR (qRT-PCR).
The analysis revealed differential expression of LEC1 but
not FUS3 within a 30 day time course of somatic embryo
development, and a significant auxin-dependent upregulation
of LEC1 was found over the time course. In contrast to
embryogenic culture, the level of LEC1 and FUS3
expression was noticeably lower in non-embryogenic callus
of Col-0 and hormonal mutants (cbp20 and axr4-1)
with low SE-efficiency. In addition, the expression profile
of LEC1 and FUS3 was followed in the embryogenic
culture derived from 35S::LEC2-GR explants. A significant
increase of LEC1 but not FUS3 activity was observed
under LEC2 overexpression induced in auxin-treated
explants. The work provides further experimental evidence
on LEC gene involvement in the embryogenic response in
Arabidopsis somatic cells, and also implicates LEC1
function in more advanced stages of SE culture in relation
to somatic embryo differentiation and development. | pl_PL |
dc.language.iso | en | pl_PL |
dc.rights | Uznanie autorstwa-Użycie niekomercyjne 3.0 Polska | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc/3.0/pl/ | * |
dc.subject | Auxin | pl_PL |
dc.subject | Embryogenic transition | pl_PL |
dc.subject | FUSCA3 | pl_PL |
dc.subject | Hormonal mutants | pl_PL |
dc.subject | LEAFY COTYLEDON1 | pl_PL |
dc.subject | LEC2 overexpression | pl_PL |
dc.title | LEAFY COTYLEDON1, FUSCA3 expression and auxin treatment in relation to somatic embryogenesis induction in Arabidopsis | pl_PL |
dc.type | info:eu-repo/semantics/article | pl_PL |
dc.identifier.doi | 10.1007/s10725-011-9585-y | - |
Pojawia się w kolekcji: | Artykuły (WNP)
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