DC pole | Wartość | Język |
dc.contributor.author | Kraut, Małgorzata | - |
dc.contributor.author | Wójcikowska, Barbara | - |
dc.contributor.author | Ledwoń, Agnieszka | - |
dc.contributor.author | Gaj, Małgorzata D. | - |
dc.date.accessioned | 2019-06-17T12:07:45Z | - |
dc.date.available | 2019-06-17T12:07:45Z | - |
dc.date.issued | 2011 | - |
dc.identifier.citation | "Acta Biologica Cracoviensia. Series Botanica" Nr 2 (2011), s. 59-67 | pl_PL |
dc.identifier.issn | 1898-0295 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.12128/9505 | - |
dc.description.abstract | To understand the molecular mechanism controlling in vitro plant morphogenesis, a culture system enabling
induction of alternative morphogenic pathways (somatic embryogenesis, SE; shoot organogenesis, ORG) in a well
defined population of somatic cells is needed. Arabidopsis is the most useful model plant for genomic studies,
but a system in which SE or ORG can be induced alternatively in the same type of explant has not been proposed.
Immature zygotic embryos (IZEs) of Arabidopsis provide the only explants with embryogenic potential,
and have been recommended for studying mechanisms of SE induced in vitro. This study was aimed at defining
culture conditions promoting induction of alternative morphogenic pathways: shoot ORG in IZE explants.
The established protocol involves pretreatment of IZE explants with liquid auxin-rich callus induction (CIM)
medium, followed by subculture on solid cytokinin-rich shoot induction medium (SIM). The method enables efficient
shoot induction in Columbia (Col-0) and Wassilewskija (Ws), genotypes commonly used in molecular studies.
During 3 weeks of culture up to 90% of Col-0 and 70% of Ws explants regenerated shoots via an indirect
morphogenic pathway. We analyzed the qRT-PCR expression patterns of the LEC (LEC1, LEC2 and FUS3) genes,
the key regulators of Arabidopsis embryogenesis, in the IZE explants induced to promote shoot ORG. The sharp
decline of LEC expression on SIM medium confirmed that culture of Arabidopsis IZE explants enables experimental
manipulation of the morphogenic response of somatic cells. A scheme illustrating various in vitro morphogenic
responses of IZEs in relation to hormonal treatment is presented. | pl_PL |
dc.language.iso | en | pl_PL |
dc.rights | Uznanie autorstwa-Użycie niekomercyjne-Bez utworów zależnych 3.0 Polska | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/pl/ | * |
dc.subject | Arabidopsis | pl_PL |
dc.subject | immature zygotic embryos | pl_PL |
dc.subject | in vitro morphogenesis | pl_PL |
dc.subject | shoot regeneration | pl_PL |
dc.title | Immature zygotic embryo cultures of Arabidopsis. Amodel system for molecular studies on morphogenic pathways induced in vitro | pl_PL |
dc.type | info:eu-repo/semantics/article | pl_PL |
dc.relation.journal | Acta Biologica Cracoviensia. Series Botanica | pl_PL |
dc.identifier.doi | 10.2478/v10182-011-0028-x | - |
Pojawia się w kolekcji: | Artykuły (WNP)
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