DC pole | Wartość | Język |
dc.contributor.author | Zelkowski, Mateusz | - |
dc.contributor.author | Zelkowska, Katarzyna | - |
dc.contributor.author | Conrad, Udo | - |
dc.contributor.author | Hesse, Susann | - |
dc.contributor.author | Lermontova, Inna | - |
dc.contributor.author | Marzec, Marek | - |
dc.contributor.author | Meister, Armin | - |
dc.contributor.author | Houben, Andreas | - |
dc.contributor.author | Schubert, Veit | - |
dc.date.accessioned | 2019-07-04T07:06:51Z | - |
dc.date.available | 2019-07-04T07:06:51Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | Frontiers in Plant Science, Vol. 10 (2019), Art. No. 774 | pl_PL |
dc.identifier.issn | 1664-462X | - |
dc.identifier.uri | http://hdl.handle.net/20.500.12128/9587 | - |
dc.description.abstract | The SMC 5/6 complex together with cohesin and condensin is a member of the
structural maintenance of chromosome (SMC) protein family. In non-plant organisms
SMC5/6 is engaged in DNA repair, meiotic synapsis, genome organization and
stability. In plants, the function of SMC5/6 is still enigmatic. Therefore, we analyzed
the crucial d-kleisin component NSE4 of the SMC5/6 complex in the model plant
Arabidopsis thaliana. Two functional conserved Nse4 paralogs (Nse4A and Nse4B)
are present in A. thaliana, which may have evolved via gene subfunctionalization.
Due to its high expression level, Nse4A seems to be the more essential gene,
whereas Nse4B appears to be involved mainly in seed development. The morphological
characterization of A. thaliana T-DNA mutants suggests that the NSE4 proteins are
essential for plant growth and fertility. Detailed investigations in wild-type and the
mutants based on live cell imaging of transgenic GFP lines, fluorescence in situ
hybridization (FISH), immunolabeling and super-resolution microscopy suggest that
NSE4A acts in several processes during plant development, such as mitosis, meiosis
and chromatin organization of differentiated nuclei, and that NSE4A operates in a
cell cycle-dependent manner. Differential response of NSE4A and NSE4B mutants
after induced DNA double strand breaks (DSBs) suggests their involvement in DNA
repair processes. | pl_PL |
dc.language.iso | en | pl_PL |
dc.rights | Uznanie autorstwa 3.0 Polska | * |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/pl/ | * |
dc.subject | Arabidopsis thaliana | pl_PL |
dc.subject | meiosis | pl_PL |
dc.subject | mitosis | pl_PL |
dc.subject | NSE4 d-kleisin | pl_PL |
dc.subject | nucleus | pl_PL |
dc.subject | phylogeny | pl_PL |
dc.subject | SMC5/6 complex | pl_PL |
dc.subject | super-resolution microscopy | pl_PL |
dc.title | Arabidopsis NSE4 Proteins Act in Somatic Nuclei and Meiosis to Ensure Plant Viability and Fertility | pl_PL |
dc.type | info:eu-repo/semantics/article | pl_PL |
dc.identifier.doi | 10.3389/fpls.2019.00774 | - |
Pojawia się w kolekcji: | Artykuły (WNP)
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