DC pole | Wartość | Język |
dc.contributor.author | Drzazga, Zofia | - |
dc.contributor.author | Michalik, Katarzyna | - |
dc.contributor.author | Halat, Tomasz | - |
dc.contributor.author | Michnik, Anna | - |
dc.contributor.author | Trzeciak, Henryk I. | - |
dc.date.accessioned | 2019-08-03T17:48:48Z | - |
dc.date.available | 2019-08-03T17:48:48Z | - |
dc.date.issued | 2010 | - |
dc.identifier.citation | Journal of Thermal Analysis and Calorimetry, Vol. 102 (2010), s, 143-148 | pl_PL |
dc.identifier.issn | 1388-6150 | - |
dc.identifier.issn | 1572-8943 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.12128/10458 | - |
dc.description.abstract | Differential scanning microcalorimetry (DSC)
and UV–VIS absorption spectroscopy were used to obtain
the characteristics of blood serum from newborn rat’ after
maternal treatment with cyclophosphamide in comparison
with control. The obtained DSC curves reveal a complex
endothermic peak due to the unfolding process of various
serum proteins. Thermal profiles and absorption spectra of
blood serum are sensitive to the age of newborns as well as
to effect of maternal administration of cyclophosphamide.
The most significant disturbances in serum proteome were
observed for 14-day old newborns. The thermodynamic
parameters: enthalpy change (DH), the normalized first
moment (M1) of the thermal transition with respect to the
temperature axis and the ratio of Cp
ex at 70 and 60 C
describing denaturation contributions of globulin forms in
respect to unliganded albumin with haptoglobin was estimated.
Moreover, the second derivative spectroscopy in the
UV region was used to resolve the complex protein spectrum.
The differences in blood serum detected by DSC and
UV–VIS confirm a potential usefulness of these methods
for diagnostic and monitoring changes with age as well as
the pathological state of blood serum. | pl_PL |
dc.language.iso | en | pl_PL |
dc.rights | Uznanie autorstwa-Użycie niekomercyjne 3.0 Polska | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc/3.0/pl/ | * |
dc.subject | Blood serum | pl_PL |
dc.subject | Cyclophosphamide | pl_PL |
dc.subject | Differential scanning microcalorimetry | pl_PL |
dc.subject | Newborn rats | pl_PL |
dc.subject | UV–VIS spectroscopy | pl_PL |
dc.title | Calorimetric and spectroscopic studies characterization of newborn rat’ blood serum after maternal administration of cyclophosphamide | pl_PL |
dc.type | info:eu-repo/semantics/article | pl_PL |
dc.identifier.doi | 10.1007/s10973-010-0969-4 | - |
Pojawia się w kolekcji: | Artykuły (WNŚiT)
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