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Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12128/10502
Title: Barley primary microRNA expression pattern is affected by soil water availability
Authors: Swida-Barteczka, Aleksandra
Kruszka, Katarzyna
Grabowska, Aleksandra
Pacak, Andrzej
Jarmolowski, Artur
Kurowska, Marzena
Szarejko, Iwona
Szweykowska-Kulinska, Zofia
Keywords: Pri-miRNA; mrRNA; Drought; Rehydration; Barley genotypes
Issue Date: 2016
Citation: Acta Biochimica Polonica, 2016, vol. 63, no. 4, p. 817-824
Abstract: MicroRNAs are short molecules of 21–24 nt in length. They are present in all eukaryotic organisms and regulate gene expression by guiding posttranscriptional silencing of mRNAs. In plants, they are key players in signal transduction, growth and development, and in response to abiotic and biotic stresses. Barley (Hordeum vulgare) is an economically important monocotyledonous crop plant. Drought is the world’s main cause of loss in cereal production. We have constructed a highthroughput Real-Time RT-qPCR platform for parallel determination of 159 barley primary microRNAs’ levels. The platform was tested for two drought-and-rehydrationtreated barley genotypes (Rolap and Sebastian). We have determined changes in the expression of primary microRNAs responding to mild drought, severe drought, and rehydration. Based on the results obtained, we conclude that alteration in the primary microRNA expression is relative to the stress’s intensity. Mild drought and rehydration mostly decrease the pri-miRNA levels in both of the tested genotypes. Severe drought mainly induces the primary microRNA expression. The main difference between the genotypes tested was a much-stronger induction of pri-miRNAs in Rolap encountering severe drought. The primary microRNAs respond dynamically to mild drought, severe drought, and rehydration treatments. We propose that some of the individual primiRNAs could be used as drought stress or rehydration markers. The usage of the platform in biotechnology is also postulated.
URI: http://hdl.handle.net/20.500.12128/10502
DOI: 10.18388/abp.2016_1352
ISSN: 0001-527X
1734-154X
Appears in Collections:Artykuły (WNP)

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