DC pole | Wartość | Język |
dc.contributor.author | Drzazga, Zofia | - |
dc.contributor.author | Ciszek, Wojciech | - |
dc.contributor.author | Binek, Mariusz | - |
dc.date.accessioned | 2019-11-20T19:44:15Z | - |
dc.date.available | 2019-11-20T19:44:15Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | Journal of Fluorescence, Vol. 29, iss. 5 (2019), s. 1257– 1263 | pl_PL |
dc.identifier.issn | 1053-0509 | - |
dc.identifier.issn | 1573-4994 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.12128/11668 | - |
dc.description.abstract | Autofluorescence of the bone extracellular matrix (ECM) has not been widely explored although the ECMplays a very important
role in bone development. In our research we focused on examining the bone matrix of very young animals due to the intense
growth process during the first month of life. Structure images and fluorescence spectra of the bone surface were carried out using
confocal fluorescence microscope Eclipse Ti-S inverted CLSM (NIKON, Japan) for compact tibia of healthy 7-, 14- and 28-dayold
rat newborns after prenatal zidovudine administration in comparison with control. Spectral features of ECM autofluorescence
were analyzed statistically by taking into consideration p < 0.05. The CLSM technique allows for simultaneous examination of
the structure and autofluorescence from selected areas of the bone surface. Excessive autofluorescence of ECM after prenatal
zidovudine administration influences bone growth incommensurably to the newborns’ age. Therefore the possibility of an
additional non-enzymatic mechanism of collagen cross-linking in the first two weeks of life of newborn rats prenatally treated
with zidovudine has been considered. Our results suggest that ECMautofluorescence can be an indicator of bone development in
the normal and pathological state. | pl_PL |
dc.language.iso | en | pl_PL |
dc.rights | Uznanie autorstwa 3.0 Polska | * |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/pl/ | * |
dc.subject | ECM | pl_PL |
dc.subject | Collagen cross-links | pl_PL |
dc.subject | Newborn rats | pl_PL |
dc.subject | Zidovudine | pl_PL |
dc.subject | Fluorescence confocal laser scanning microscopy | pl_PL |
dc.title | Prenatal Zidovudine Treatment Modifies Early Development of Rat Osteoid – Confocal Microspectroscopy Analysis | pl_PL |
dc.type | info:eu-repo/semantics/article | pl_PL |
dc.identifier.doi | 10.1007/s10895-019-02429-6 | - |
Pojawia się w kolekcji: | Artykuły (WNŚiT)
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