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Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12128/11691
Title: Field cress genome mapping: Integrating linkage and comparative maps with cytogenetic analysis for rDNA carrying chromosomes
Authors: Desta, Zeratsion Abera
Kolano, Bożena
Shamim, Zeeshan
Armstrong, Susan J.
Rewers, Monika
Śliwińska, Estera
Kushwaha, Kumar Kushwaha
Parkin, Isobel A.P.
Ortiz, Rodomiro
de Koning, Dirk-Jan
Keywords: Cytogenetic analysis; Lepidium campestre; rDNA; Genome analysis; Field cress; Genetic map
Issue Date: 2019
Citation: Scientific Reports (Nature Publishing Group), (2019), iss. 9., art no. 17028
Abstract: Field cress (Lepidium campestre L.), despite its potential as a sustainable alternative oilseed plant, has been underutilized, and no prior attempts to characterize the genome at the genetic or molecular cytogenetic level have been conducted. Genetic maps are the foundation for anchoring and orienting annotated genome assemblies and positional cloning of candidate genes. Our principal goal was to construct a genetic map using integrated approaches of genetic, comparative and cytogenetic map analyses. In total, 503 F2 interspecific hybrid individuals were genotyped using 7,624 single nucleotide polymorphism markers. Comparative analysis demonstrated that ~57% of the sequenced loci in L. campestre were congruent with Arabidopsis thaliana (L.) genome and suggested a novel karyotype, which predates the ancestral crucifer karyotype. Aceto-orcein chromosome staining and fluorescence in situ hybridization (FISH) analyses confirmed that L. campestre, L. heterophyllum Benth. and their hybrids had a chromosome number of 2n = 2x = 16. Flow cytometric analysis revealed that both species possess 2C roughly 0.4 picogram DNA. Integrating linkage and comparative maps with cytogenetic map analyses assigned two linkage groups to their particular chromosomes. Future work could incorporate FISH utilizing A. thaliana mapped BAC clones to allow the chromosomes of field cress to be identified reliably.
URI: http://hdl.handle.net/20.500.12128/11691
DOI: 10.1038/s41598-019-53320-0
ISSN: 2045-2322
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