DC pole | Wartość | Język |
dc.contributor.author | Wojakowska, Anna | - |
dc.contributor.author | Pietrowska, Monika | - |
dc.contributor.author | Widlak, Piotr | - |
dc.contributor.author | Dobrowolski, Dariusz | - |
dc.contributor.author | Wylęgała, Edward | - |
dc.contributor.author | Tarnawska, Dorota | - |
dc.date.accessioned | 2020-07-07T08:00:27Z | - |
dc.date.available | 2020-07-07T08:00:27Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | Molecules, Vol. 25 (2020), Art. No. 2933 | pl_PL |
dc.identifier.issn | 1420-3049 | - |
dc.identifier.issn | 1420-3049 | - |
dc.identifier.issn | 10.3390/molecules25122933 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.12128/15009 | - |
dc.description.abstract | The molecular etiology of keratoconus (KC), a pathological condition of the human
cornea, remains unclear. The aim of this work was to perform profiling of metabolites and
identification of features discriminating this pathology from the normal cornea. The combination
of gas chromatography and mass spectrometry (GC/MS) techniques has been applied for profiling
and identification of metabolites in corneal buttons from 6 healthy controls and 7 KC patients.
An untargeted GC/MS-based approach allowed the detection of 377 compounds, including 46
identified unique metabolites, whose levels enabled the separation of compared groups of samples
in unsupervised hierarchical cluster analysis. There were 13 identified metabolites whose levels
di erentiated between groups of samples. Downregulation of several carboxylic acids, fatty acids,
and steroids was observed in KC when compared to the normal cornea. Metabolic pathways
associated with compounds that discriminated both groups were involved in energy production,
lipid metabolism, and amino acid metabolism. An observed signature may reflect cellular processes
involved in the development of KC pathology, including oxidative stress and inflammation. | pl_PL |
dc.language.iso | en | pl_PL |
dc.rights | Uznanie autorstwa 3.0 Polska | * |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/pl/ | * |
dc.subject | keratoconus | pl_PL |
dc.subject | cornea | pl_PL |
dc.subject | ocular metabolomics | pl_PL |
dc.subject | GC/MS profiling | pl_PL |
dc.title | Metabolomic Signature Discriminates Normal Human Cornea from Keratoconus - A Pilot GC/MS Study | pl_PL |
dc.type | info:eu-repo/semantics/article | pl_PL |
Pojawia się w kolekcji: | Artykuły (WNŚiT)
|