DC pole | Wartość | Język |
dc.contributor.author | Korzuch, Julia | - |
dc.contributor.author | Rak, Monika | - |
dc.contributor.author | Balin, Katarzyna | - |
dc.contributor.author | Zubko, Maciej | - |
dc.contributor.author | Dulski, Mateusz | - |
dc.contributor.author | Głowacka, Olga | - |
dc.contributor.author | Musioł, Robert | - |
dc.contributor.author | Serda, Maciej | - |
dc.contributor.author | Madeja, Zbigniew | - |
dc.date.accessioned | 2021-06-02T09:39:51Z | - |
dc.date.available | 2021-06-02T09:39:51Z | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | "Scientific Reports" (2021), Vol. 11, art. no. 10565, s. 1-9 | pl_PL |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.12128/20336 | - |
dc.description.abstract | This paper presents two water-soluble fullerene nanomaterials (
HexakisaminoC60 and
monoglucosamineC60,
which is called here JK39) that were developed and synthesized as nonviral
siRNA transfection nanosystems. The developed two-step Bingel–Hirsch reaction enables
the chemical modification of the fullerene scaffold with the desired bioactive fragments such as
d-glucosamine while keeping the crucial positive charged ethylenediamine based malonate. The
ESI–MS and 13C-NMR analyses of JK39 confirmed its high Th
symmetry, while X-ray photoelectron
spectroscopy revealed the presence of nitrogen and oxygen-containing C–O or C–N bonds. The
efficiency of both fullerenes as siRNA vehicles was tested in vitro using the prostate cancer cell line
DU145 expressing the GFP protein. The HexakisaminoC60
fullerene was an efficient siRNA transfection
agent, and decreased the GFP fluorescence signal significantly in the DU145 cells. Surprisingly,
the glycofullerene JK39 was inactive in the transfection experiments, probably due to its high zeta
potential and the formation of an extremely stable complex with siRNA. | pl_PL |
dc.language.iso | en | pl_PL |
dc.rights | Uznanie autorstwa 3.0 Polska | * |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/pl/ | * |
dc.subject | cancer | pl_PL |
dc.subject | chemical biology | pl_PL |
dc.subject | chemistry | pl_PL |
dc.subject | drug discovery | pl_PL |
dc.subject | medicinal chemistry | pl_PL |
dc.subject | nanomedicine | pl_PL |
dc.subject | nanoscience and technology | pl_PL |
dc.subject | siRNA | pl_PL |
dc.subject | water-soluble [60]fullerenes | pl_PL |
dc.subject | prostate cancer | pl_PL |
dc.title | Towards water-soluble [60]fullerenes for the delivery of siRNA in a prostate cancer model | pl_PL |
dc.type | info:eu-repo/semantics/article | pl_PL |
dc.identifier.doi | 10.1038/s41598-021-89943-5, | - |
Pojawia się w kolekcji: | Artykuły (WNŚiT)
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