Skip navigation

Please use this identifier to cite or link to this item:
Title: Blood serum denaturation profile examined by differential scanning calorimetry reflects the effort put into ultramarathon by amateur long-distance runners
Authors: Michnik, Anna
Sadowska-Krępa, Ewa
Kiełboń, Agnieszka
Duch, Klaudia
Bańkowski, Sebastian
Keywords: Amateur runners; Differential scanning calorimetry (DSC); Human blood serum; Protein thermal denaturation; Ultramarathon run
Issue Date: 2021
Citation: "Journal of Thermal Biology" (2021), vol. 99, art. no. 103013, s. 1-7
Abstract: The impact of participation in the ultramarathon on the health and mental and physical condition is very complex. Undoubtedly, exercise brings many benefits but also involves health risks. Especially such an extreme effort as the one associated with finishing the ultramarathon run, can be dangerous to the health of the runner. With the variety of possible biomarkers of excessive fatigue that threaten health and life, a question arises which of them are the best and which should be considered in amateur long-distance runners showing particularly high individual variability. In this study differential scanning calorimetry (DSC) has been applied to show the overall effect of the 12-h run on blood sera of participants. Serum samples were obtained from the blood of ten male amateur long-distance runners, collected before and immediately after the run. Distinct changes in the shape of DSC curves have been observed for serum after finishing the run relative to pre-race serum. Statistically significant differences between stages “before” and “after” ultramarathon running have been found for parameters of the endothermic transition associated with denaturation of serum proteins. An increase in the temperature (from 70.9 ± 0.9 to 75.8 ± 2.9 ◦C) and excess heat capacity (from 0.859 ± 0.201 to 1.102 ± 0.226 Jg-1 ◦C-1) at peak maximum, the enthalpy of serum denaturation (from 18.55 ± 6.52 to 22.08 ± 5.61 Jg-1) and the first moment of the thermal transition with respect to the temperature (from the value of 67.0 ± 2.1 to 72.6 ± 2.1 ◦C) has been observed. These results show a clear impact of running an ultramarathon on the participant’s blood serum.
DOI: 10.1016/j.jtherbio.2021.103013
ISSN: 0306-4565
Appears in Collections:Artykuły (WNŚiT)

Files in This Item:
File Description SizeFormat 
Michnik_blood_serum_denaturation_profile.pdf1,59 MBAdobe PDFView/Open
Show full item record

Uznanie autorstwa - użycie niekomercyjne, bez utworów zależnych 3.0 Polska Creative Commons License Creative Commons