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Zastosuj identyfikator do podlinkowania lub zacytowania tej pozycji: http://hdl.handle.net/20.500.12128/257
Tytuł: Susceptibility of staphylococcus aureus clinical isolates to propolis extract alone or in combination with antimicrobial drugs
Autor: Wojtyczka, Robert D.
Dziedzic, Arkadiusz
Idzik, Danuta
Kępa, Małgorzata
Kubina, Robert
Kabała-Dzik, Agata
Smoleń-Dzirba, Joanna
Stojko, Jerzy
Sajewicz, Mieczysław
Wąsik, Tomasz J.
Słowa kluczowe: Ethanolic extract of Polish propolis (EEPP); Methicillin-resistant S. aureus (MRSA); Methicillin-sensitive S. aureus (MSSA)
Data wydania: 2013
Źródło: Molecules (Basel), vol. 18, iss. 8 (2013), s. 9623-9640
Abstrakt: The objective of this study was to assess in vitro the antimicrobial activity of ethanolic extract of Polish propolis (EEPP) against methicillin-sensitive Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA) clinical isolates. The combined effect of EEPP and 10 selected antistaphylococcal drugs on S. aureus clinical cultures was also investigated. EEPP composition was analyzed by a High Performance Liquid Chromatography (HPLC) method. The flavonoid compounds identified in Polish Propolis included flavones, flavonones, flavonolols, flavonols and phenolic acids. EEPP displayed varying effectiveness against twelve S. aureus strains, with minimal inhibitory concentration (MIC) within the range from 0.39 to 0.78 mg/mL, determined by broth microdilution method. The average MIC was 0.54 ± 0.22 mg/mL, while calculated MIC50 and MIC90 were 0.39 mg/mL and 0.78 mg/mL, respectively. The minimum bactericidal concentration (MBC) of the EEPP ranged from 0.78 to 3.13 mg/mL. The in vitro combined effect of EEPP and 10 antibacterial drugs was investigated using disk diffusion method-based assay. Addition of EEPP to cefoxitin (FOX), clindamycin (DA), tetracycline (TE), tobramycin (TOB), linezolid (LIN), trimethoprim+sulfamethoxazole (SXT), penicillin (P), erythromycin (E) regimen, yielded stronger, cumulative antimicrobial effect, against all tested S. aureus strains than EEPP and chemotherapeutics alone. In the case of ciprofloxacin (CIP) and chloramphenicol (C) no synergism with EEPP was observed.
URI: http://hdl.handle.net/20.500.12128/257
DOI: 10.3390/molecules18089623
ISSN: 1420-3049
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