http://hdl.handle.net/20.500.12128/8203
Tytuł: | Stability and instability processes in the calli of Fagopyrum tataricum that have different morphogenic potentials |
Autor: | Betekhtin, Alexander Piński, Artur Milewska-Hendel, Anna Kurczyńska, Ewa Hasterok, Robert |
Słowa kluczowe: | Fagopyrum tataricum; Ethylene; Calli; Morphogenic potential; Hydroxyproline-rich glycoproteins; The maintenance of telomere; DNA methyltransferases; Trichostatin A |
Data wydania: | 14-lut-2019 |
Źródło: | “Plant Cell, Tissue and Organ Culture” (Vol. 137, iss. 2 (2019), s. 343–357) |
Abstrakt: | The morphogenic callus (MC) of Fagopyrum tataricum contains a large amount of flavonoids, especially rutin, and exhibits a high level of antioxidant activity. A non-morphogenic callus (NC) may appear on the surface of the MC after two to three years of cultivation and is then subjected to a consistently high level of oxidative stress. The elucidation of the molecular background of this instability is essential for gaining a better understanding of the somaclonal variation mechanisms in tissue cultures that have different morphogenic potentials. Thus, in this study we show that continuous oxidative stress in a NC might be connected with a rapid senescence process and as a result, in the upregulation of the genes that are connected with the telomere complexity, ethylene biosynthesis and the expression of DNA methyltransferases. Moreover, we analysed the presence of the hydroxyproline-rich glycoproteins in the calli and demonstrated the differences between the MC and NC. The LM2 antibody can be useful as a marker of the cells in the MC that are embryogenically determined, while the MAC207 antibody seems to be a positive marker of a MC as its signal was absent in the NC. This study also provides the first report on the effect of trichostatin A on the DNA methyltransferases and demethylases in a MC. |
URI: | http://hdl.handle.net/20.500.12128/8203 |
DOI: | 10.1007/s11240-019-01575-w |
ISSN: | 1573-5044 |
Pojawia się w kolekcji: | Artykuły (WNP) |
Plik | Opis | Rozmiar | Format | |
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Betekhtin_Stability_and_instability_processes_in_the_calli_of.pdf | 2,2 MB | Adobe PDF | Przejrzyj / Otwórz |
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