DC pole | Wartość | Język |
dc.contributor.author | Dołowy, Małgorzata | - |
dc.contributor.author | Kozik, Violetta | - |
dc.contributor.author | Bąk, Andrzej | - |
dc.contributor.author | Jampilek, Josef | - |
dc.contributor.author | Barbusiński, Krzysztof | - |
dc.contributor.author | Thomas, Maciej | - |
dc.contributor.author | Pyka-Pająk, Alina | - |
dc.date.accessioned | 2019-03-19T10:30:14Z | - |
dc.date.available | 2019-03-19T10:30:14Z | - |
dc.date.issued | 2017 | - |
dc.identifier.citation | EPJ Web of Conferences, Vol. 22, iss. 11 (2017), art. no 1888 | pl_PL |
dc.identifier.issn | 1420-3049 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.12128/8605 | - |
dc.description.abstract | A rapid, simple to use and low-cost thin-layer chromatographic procedure in normal phase
system with densitometric detection at 246 nm was carefully validated according to the International
Conference on Harmonisation (ICH) guidelines for assay of clobetasol propionate in topical solution
containing clobetasol propionate in quantity 0.50 mg/mL. The adopted thin-layer chromatographic
(TLC)-densitometric procedure could effectively separate clobetasol propionate from its related
compound, namely clobetasol. It is linear for clobetasol propionate in the range of 0.188 5 g/spot.
The limit of detection (LOD) and limit of quantification (LOQ) value is 0.061 and 0.186 g/spot,
respectively. Accuracy of proposed procedure was evaluated by recovery test. The mean recovery
of studied clobetasol propionate ranges from 98.7 to 101.0%. The coefficient of variation (CV, %)
obtained during intra-day and inter-day studies, which was less than 2% (0.40 1.17%), confirms
the precision of described method. The assay value of clobetasol propionate is consistent with the
pharmacopoeial requirements. In conclusion, it can be suitable as a simple and economic procedure
for routine quality control laboratories of clobetasol propionate in topical solution. | pl_PL |
dc.language.iso | en | pl_PL |
dc.rights | Uznanie autorstwa 3.0 Polska | * |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/pl/ | * |
dc.subject | clobetasol | pl_PL |
dc.subject | clobetasol propionate | pl_PL |
dc.subject | topical steroids | pl_PL |
dc.subject | TLC-densitometry | pl_PL |
dc.subject | validation | pl_PL |
dc.title | Rapid and simple TLC-densitometric method for assay of clobetasol propionate in topical solution | pl_PL |
dc.type | info:eu-repo/semantics/article | pl_PL |
dc.identifier.doi | 10.3390/molecules22111888 | - |
Pojawia się w kolekcji: | Artykuły (WNŚiT)
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