http://hdl.handle.net/20.500.12128/221
Title: | Ileal transposition in rats influenced glucose metabolism and HSP70 levels |
Authors: | Stygar, Dominika Sawczyn, Tomasz Skrzep-Poloczek, Bronisława Karcz-Socha, Iwona Doleżych, Bogdan Zawisza-Raszka, Agnieszka Augustyniak, Maria Żwirska-Korczala, Krystyna Karcz, Wojciech Konrad |
Keywords: | HSP70; Ileal transposition; Metabolic surgery; Obesity surgery; Type 2 diabetes mellitus |
Issue Date: | 2015 |
Citation: | Open Life Sciences, Vol. 10, iss. 1 (2015), s. 278-284 |
Abstract: | Objective: Ileal transposition procedure (IT), in combination with sleeve gastrectomy, is widely used to induce diabetes remission and to control related metabolic abnormalities. A transposition of a long segment of distal ileum in obese Zucker rats improved glucose tolerance 6 months after IT. The premise of our study was to to examine the long - term effects of ileum transposition on the liver glycolytic enzymes content in a euglycemic group of operated Zucker rats. Methods: Twenty male Zucker rats underwent either the transposition of 50% distal ileum or a sham surgery. Six months after surgery, liver tissue concentrations of glycogen synthase kinase alpha (GSK-3α), glucose 6-phosphatase (G6PC), glycogen phosphorylase (PYGM) and phosphofructokinase (PFK) and HSP70 were assessed by immunoenzymatic methods. Results: HSP70 values were significantly higher in the IT group compared to SHAM. G6PC liver concentrations in the IT group were almost 1.45-fold lower than in the SHAM operated rats. Statistical analyses (F-test) showed HSP70 levels were significantly related to caveolin-1and SHAM group. Conclusions: Lowered glycolytic enzyme concentrations assessed in the liver suggest positive effects on glucose metabolism in long-term observations. |
URI: | http://hdl.handle.net/20.500.12128/221 |
DOI: | 10.1515/biol-2015-0029 |
ISSN: | 2391-5412 |
Appears in Collections: | Artykuły (WNP) |
File | Description | Size | Format | |
---|---|---|---|---|
Stygar_Ileal_transposition_in_rats_influenced_glucose_metabolism_and_HSP70_levels.pdf | 753,69 kB | Adobe PDF | View/Open |
Uznanie autorstwa - użycie niekomercyjne, bez utworów zależnych 3.0 Polska Creative Commons License