| Abstrakt: | The doctoral dissertation is divided into two parts: theoretical and experimental. In the
theoretical part, primary and secondary plant metabolites are discussed and individual groups
of phytochemical compounds are characterized. The following chapters are devoted to the
compounds from the group of anthocyanins, betacyanins and neolignans. The experimental
part includes descriptions of the research and analyses carried out using thin-layer
chromatography (TLC and HPTLC methods), scanning densitometry, UV-Vis
spectrophotometry and mass spectrometry (TLC-MS). The aim of the study was to develop
new conditions for the chromatographic separation of compounds, as well as to apply the
method for their qualitative and quantitative determination. The first stage is focused on the
analysis of selected anthocyanins: cyanin, keracyanin, delphinidin and pelargonidin.
In addition, a method of triple chromatographic development for the separation
of anthocyanins was carried out. The tested compounds were determined in food samples.
The next stage of research focuses on betacyanins, including betanin. Horizontal separation
of betacyanin pigments was observed during the chromatographic analysis, which
is a "fingerprint" of those compounds. The last part of the experimental concerns compounds
from the group of neolignans: magnolol and honokiol, which were determined in samples
of dietary supplements, and their antioxidant and antibacterial properties were examined. |