http://hdl.handle.net/20.500.12128/23475
Title: | New Diagnostic Tool for Ion Channel Activity Hidden Behind the Dwell-Time Correlations |
Authors: | Borys, Przemysław Trybek, Paulina Dworakowska, Beata Bednarczyk, Piotr Wawrzkiewicz-Jałowiecka, Agata |
Keywords: | Cluster chemistry; Conformation; Membranes; Plasma membrane; Polarization |
Issue Date: | 2022 |
Citation: | "Journal of Physical Chemistry B" (2022) |
Abstract: | The patch-clamp technique is a powerful tool that allows for a long observation of transport protein activity in real time. Experimental traces of single-channel currents can be considered as a record of the channel’s conformational switching related to its activation and gating. In this work, we present a mathematically simple method of patch-clamp data analysis that assesses the connectivity and occupancy of distinct conformational substates of the channel. The proposed approach appears to be a big step forward due to its possible applications in the determination of channel substates related to disease and in the analysis of drug−channel interactions on the level of repetitive sequences of channel conformations. This is especially important in cases when molecular dynamics docking is impossible and Markovian modeling requires ambiguous optimization tasks. |
URI: | http://hdl.handle.net/20.500.12128/23475 |
DOI: | 10.1021/acs.jpcb.2c02272 |
ISSN: | 1520-5207 |
Appears in Collections: | Artykuły (WNŚiT) |
File | Description | Size | Format | |
---|---|---|---|---|
Borys_Trybek_new_diagnostic_tool_for_ion.pdf | 4,24 MB | Adobe PDF | View/Open |
Uznanie Autorstwa 3.0 Polska Creative Commons License