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Title: Oxysterols Increase Inflammation, Lipid Marker Levels and Reflect Accelerated Endothelial Dysfunction in Experimental Animals
Authors: Wielkoszyński, Tomasz
Zalejska-Fiolka, Jolanta
Strzelczyk, Joanna K.
Owczarek, Aleksander J.
Cholewka, Armand
Furmański, Marcin
Stanek, Agata
Keywords: Hypercholesterolemic Rabbits; Plasma-Concentration; Atherosclerosis; Atorvastatin; Proliferation; Homocysteine; Secretion
Issue Date: 2018
Citation: Mediators of Inflammation, Art. ID 2784701, (2018), s. 1-19
Abstract: Objective. Oxidized cholesterol derivatives are thought to exert atherogenic effect thus adversely affecting vascular endothelium. The aim of the study was to assess the effect of 5α,6α-epoxycholesterol on experimentally induced hypercholesterolemia in rabbits, and the levels of homocysteine (HCY), asymmetric dimethylarginine (ADMA), paraoxonase-1 (PON-1), and inflammatory parameters (IL-6, TNF-α, CRP). Material and methods. The rabbits were divided into 3 groups, 8 animals each, and fed with basic fodder (C), basic fodder plus cholesterol (Ch) or basic fodder plus 5α,6α-epoxycholesterol, and unoxidized cholesterol (ECh). Serum concentrations of studied parameters were determined at 45-day intervals. The study was continued for six months. Results. We demonstrated that adding 5α,6α-epoxycholesterol to basic fodder significantly affected lipid status of the experimental animals, increasing total cholesterol and LDL cholesterol levels, as well as HCY and ADMA levels, whilst leaving the PON-1 activity unaffected. Additionally, the ECh group presented with significantly higher concentrations of inflammatory biomarkers (IL-6, TNF-α, and CRP). In the Ch group, lower yet significant (as compared to the C group) changes of levels of studied parameters were observed. Conclusion. Exposure of animals with experimentally induced hypercholesterolemia to 5α,6α-epoxycholesterol increases dyslipidaemia, endothelial dysfunction, and inflammatory response.
DOI: 10.1155/2018/ 2784701
ISSN: 0962-9351
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