http://hdl.handle.net/20.500.12128/3214
Title: | Oxysterols Increase Inflammation, Lipid Marker Levels and Reflect Accelerated Endothelial Dysfunction in Experimental Animals |
Authors: | Wielkoszyński, Tomasz Zalejska-Fiolka, Jolanta Strzelczyk, Joanna K. Owczarek, Aleksander J. Cholewka, Armand Furmański, Marcin Stanek, Agata |
Keywords: | Hypercholesterolemic Rabbits; Plasma-Concentration; Atherosclerosis; Atorvastatin; Proliferation; Homocysteine; Secretion |
Issue Date: | 2018 |
Citation: | Mediators of Inflammation, Art. ID 2784701, (2018), s. 1-19 |
Abstract: | Objective. Oxidized cholesterol derivatives are thought to exert atherogenic effect thus adversely affecting vascular endothelium. The aim of the study was to assess the effect of 5α,6α-epoxycholesterol on experimentally induced hypercholesterolemia in rabbits, and the levels of homocysteine (HCY), asymmetric dimethylarginine (ADMA), paraoxonase-1 (PON-1), and inflammatory parameters (IL-6, TNF-α, CRP). Material and methods. The rabbits were divided into 3 groups, 8 animals each, and fed with basic fodder (C), basic fodder plus cholesterol (Ch) or basic fodder plus 5α,6α-epoxycholesterol, and unoxidized cholesterol (ECh). Serum concentrations of studied parameters were determined at 45-day intervals. The study was continued for six months. Results. We demonstrated that adding 5α,6α-epoxycholesterol to basic fodder significantly affected lipid status of the experimental animals, increasing total cholesterol and LDL cholesterol levels, as well as HCY and ADMA levels, whilst leaving the PON-1 activity unaffected. Additionally, the ECh group presented with significantly higher concentrations of inflammatory biomarkers (IL-6, TNF-α, and CRP). In the Ch group, lower yet significant (as compared to the C group) changes of levels of studied parameters were observed. Conclusion. Exposure of animals with experimentally induced hypercholesterolemia to 5α,6α-epoxycholesterol increases dyslipidaemia, endothelial dysfunction, and inflammatory response. |
URI: | http://hdl.handle.net/20.500.12128/3214 |
DOI: | 10.1155/2018/ 2784701 |
ISSN: | 0962-9351 |
Appears in Collections: | Artykuły (WNŚiT) |
File | Description | Size | Format | |
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Wielkoszynski_Oxysterols_increase_inflammation_lipid_marker_levels.pdf | 1,89 MB | Adobe PDF | View/Open |
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